Recombinant Human STK24/MST3 Protein (His Tag)(Active)

Size: 50μg

Catalogue Number: PKSH030330-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: STK24; MST3

Target Symptom: HEL-S-95; MST3; MST3B; RP11-111L24.5; STE20; STK3

Target Species: Human

Expression Host: Baculovirus-Insect Cells

Application: Enzyme

Fusion Tag: N-His

UNIProt ID: Q9Y6E0

Accession: Q9Y6E0-2

Background: MST3 encodes a serine/threonine protein kinase that functions upstream of mitogen-activated protein kinase (MAPK) signaling. The encoded protein is cleaved into two chains by caspases; the N-terminal fragment (MST3/N) translocates to the nucleus and promotes programmed cells death. There is a pseudogene for this gene on chromosome X. Alternative splicing results in multiple transcript variants.

Activity: The specific activity was determined to be 62 nmol/min/mg using synthetic PKCtide peptide (ERMRPRKRQGSVRRRV) as substrate.

Sequence: Met 1-His 431

Purity: > 82 % as determined by reducing SDS-PAGE.

Formulation: Supplied as sterile solution of 50mM Tris, 100mM NaCl, pH 8.0, 20% glycerol, 0.1mM EGTA, 0.1mM EDTA, 0.25mM DTT
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 50.3 kDa

Observed MW: 55 kDa

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Research Use Only